Principle app medium
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Avoid whipping air into suspension during the collection and mixing. Prepare 1000 ml of a uniform suspension of fresh eggs under aseptic conditions.Heat if necessary to dissolve the medium completely.Dissolve 37.3 gm of the medium in 600 ml of distilled water containing 12 ml of glycerol.It is also used for differentiating different species of mycobacterium (by colony morphology, growth rate, biochemical characteristics and microscopy).
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It is used for testing antibiotic susceptibility of isolates.It is used for the diagnosis of Mycobacterial infections.Glycerol serves as a carbon source and is favorable to the growth of the human type tubercle bacillus while being unfavorable to the bovine type. When heated, the egg albumin coagulates, thus providing a solid surface for inoculation. Egg Suspension provide fatty acids and protein required for the metabolism of mycobacteria. Malachite green, prevent the growth of the majority of contaminants surviving decontamination of the specimen while encouraging the growth of Mycobacteria. Monopotassium Phosphate and Magnesium Sulfate enhance organism growth and act as buffers. L-Asparagine and Potato Flour are sources of nitrogen and vitamins. bovis, glycerol is omitted and sodium pyruvate is added. Composition of LJ Medium Ingredientsįor cultivation of M. This formulation also encourages the earliest possible growth of mycobacteria. Jensen’s version eliminates congo red and uses a moderate concentration of malachite green to prevent growth of the majority of contaminants surviving decontamination of the specimen. It was developed by Lowenstein who incorporated congo red and malachite green to inhibit unwanted bacteria. The present formulation, a glycerated egg-based medium, is based upon Jensen’s modification. Lowenstein-Jensen (LJ) is the selective medium which is used for the cultivation and isolation of Mycobacterium species.